Journal: Cell

Article Title: The Parkinson’s disease protein alpha-synuclein is a modulator of Processing-bodies and mRNA stability

doi: 10.1016/j.cell.2022.05.008

Figure Lengend Snippet: Key resources table

Article Snippet: BCA Protein Assay Kit , Pierce , 23225.

Techniques: Western Blot, Virus, Recombinant, Protease Inhibitor, Lysis, Magnetic Beads, Membrane, Transfection, Expressing, Bicinchoninic Acid Protein Assay, Silver Staining, In Situ, Sample Prep, Luciferase, Reporter Assay, Multiplex sample analysis, Biomarker Discovery, Marker, Generated, Software, Mass Spectrometry, Imaging

Journal: International Journal of Molecular Sciences

Article Title: Colonic Mucosal Immune Activation in Mice with Ovalbumin-Induced Allergic Airway Disease: Association between Allergic Airway Disease and Irritable Bowel Syndrome

doi: 10.3390/ijms23010181

Figure Lengend Snippet: The results of cytokine ELISA assay. T-helper 1 (Th1) (IL-1β, interferon [IFN]-γ, and tumor necrosis factor [TNF]-α), Th2 (IL-4, IL-5 and IL-10), and proinflammatory cytokine (IL-6) levels were measured using ELISA with a multiplex protein assay kit. * p < 0.05, ** p = 0.064.

Article Snippet: A multiplex protein assay kit (Bio-Rad, Hercules, CA, USA) was used to measure the mucosal levels of Th1 (IFN-γ, IL-1β and TNF-α), Th2 (IL-4, IL-5 and IL-10), and proinflammatory cytokines (IL-6).

Techniques: Enzyme-linked Immunosorbent Assay, Multiplex Assay

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Identification of Tengfu Jiangya Tablet Target Biomarkers with Quantitative Proteomic Technique

doi: 10.1155/2017/7594805

Figure Lengend Snippet: Bioinformatics analysis of differentially expressed proteins. GO analysis results showed cellular component (a), molecular function (b), and biological process (c). STRING analysis indicated visualization of protein-protein interactions for 20 candidate proteins (d). The notes for different color lines were in the bottom of (d). In addition, 5 candidate biomarkers verified by ELISA and Western Blot were marked with ∗ .

Article Snippet: The concentration of bradykinin, which was the downstream product of KNG1, was also detected with ELISA kit (Cusabio Biotech, Wuhan, Hubei, China).

Techniques: Protein-Protein interactions, Enzyme-linked Immunosorbent Assay, Western Blot

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Identification of Tengfu Jiangya Tablet Target Biomarkers with Quantitative Proteomic Technique

doi: 10.1155/2017/7594805

Figure Lengend Snippet: The molecular function, biological process, and expression levels of 20 candidate serum proteins quantified by iTRAQ technique.

Article Snippet: The concentration of bradykinin, which was the downstream product of KNG1, was also detected with ELISA kit (Cusabio Biotech, Wuhan, Hubei, China).

Techniques: Expressing, Multiplex sample analysis, Binding Assay, Chemotaxis Assay, Activity Assay, Antioxidant Activity Assay, Protein Binding, Transduction, Activation Assay

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Identification of Tengfu Jiangya Tablet Target Biomarkers with Quantitative Proteomic Technique

doi: 10.1155/2017/7594805

Figure Lengend Snippet: Validation of Kininogen-1, Keratin 1, Myeloperoxidase, Retinol binding protein 4, Serum amyloid A protein, and bradykinin in serum. (a) Levels of these candidate biomarkers and downstream substance were measured by ELISA in serum of EH patients ( n = 30) and TJT treated patients ( n = 30). p values were calculated with ANOVA test ( ∗ p = 0.000). (b) With Western Blot results, the expression of Kininogen 1 and Retinol binding protein 4 was significantly differentiated between two groups. Transferrin was applied as loading control.

Article Snippet: The concentration of bradykinin, which was the downstream product of KNG1, was also detected with ELISA kit (Cusabio Biotech, Wuhan, Hubei, China).

Techniques: Biomarker Discovery, Binding Assay, Enzyme-linked Immunosorbent Assay, Western Blot, Expressing, Control

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Identification of Tengfu Jiangya Tablet Target Biomarkers with Quantitative Proteomic Technique

doi: 10.1155/2017/7594805

Figure Lengend Snippet: This map directly summarized the pathway of 5 target proteins (green ovals). The arrowheads represented variation trends of target protein after TJT treatment. KNG1, KRT1, and MPO were involved in bradykinin production; meanwhile MPO and RBP4 played vital role in vascular oxidative and inflammatory injury. SAA was an acute-phase marker for vascular injury.

Article Snippet: The concentration of bradykinin, which was the downstream product of KNG1, was also detected with ELISA kit (Cusabio Biotech, Wuhan, Hubei, China).

Techniques: Marker